United States Department of Agriculture patents. 2003 Jan. 7, no. US 6,503,698 B1, 14 p.
NALT Subjects:
swine embryo (animal) blastocyst cryopreservation new methods centrifugation lipids tissue distribution zona pellucida embryo transfer patents USDA morula United States
Other Subjects:
vitrification
Issue Date:
7-Jan-2003
Abstract:
A method for the cryopreservation of zona pellucida-intact, early-stage swine embryos, morulae and blastocysts includes centrifuging the embryos prior to cryopreservation in order to remove intracytoplasmic lipids. The lipids are localized external to the embryo proper inside the zona pellucida, thus neutralizing harmful effects which prevent subsequent embryological development. The embryos may be cryopreserved by vitrification or conventional freezing. After recovery and rehydration, zona pellucidae are removed, and intact, viable embryos are transferred to recipient females.
